cat 340 07971  (Dojindo Labs)

Journal: eLife

Article Title: Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation

doi: 10.7554/eLife.89724

Figure Lengend Snippet: ( A ) Phase-contrast images of suspension-cultured hiPSCs (WTC11 line) on day 10 (passage 2). Cells were culture in StemFit AK02N medium with no supplement (left), with LY333531 + IWR-1-endo (middle) for 10 days, with Staurosporine for 2 hours. Scale bars: 200 µm. ( B ) Evaluation of apoptotic cells by flow cytometry using Annexin V (Alexa Fluor 680) conjugates. ( C ) Bar graph indicating Annexin V-positive cells (%) in each culture conditions. ( D ) Bar graph indicating double positive cells for Annexin V and DAPI (%) in each culture conditions. Data are presented as mean ± SE (n = 3). Statistical analysis was performed using by one-way ANOVA and Tukey’s tests for all graphs. p-values <0.05 were considered statistically significant. ** in the graphs indicate p<0.01.

Article Snippet: The dissociated cells were aliquoted into 1 × 10 5 cells/100 μL with a binding buffer consisting of 10 mM HEPES, 140 mM NaCl, 2.5 mM CaCl 2 , then added with 5 μL Annexin V (Alexa Fluor 680) conjugates (Cat#A35109, Thermo Fisher Scientific) and 1 μL DAPI solution (Cat#340-07971, FUJIFILM Wako Pure Chemical Corporation).

Techniques: Suspension, Cell Culture, Flow Cytometry

Journal: eLife

Article Title: Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation

doi: 10.7554/eLife.89724

Figure Lengend Snippet:

Article Snippet: The dissociated cells were aliquoted into 1 × 10 5 cells/100 μL with a binding buffer consisting of 10 mM HEPES, 140 mM NaCl, 2.5 mM CaCl 2 , then added with 5 μL Annexin V (Alexa Fluor 680) conjugates (Cat#A35109, Thermo Fisher Scientific) and 1 μL DAPI solution (Cat#340-07971, FUJIFILM Wako Pure Chemical Corporation).

Techniques: Recombinant, Suspension, Plasmid Preparation